![]() We include sufficient reagents to perform 100 assays and a common ROS inducer as control for the measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy. Reactive oxygen species (ROS) also play a role in a variety of processes, including cell growth, production, and comeback to biotic and abiotic environmental inducements, as well as programmed cell death and signal transduction. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. ROS (Reactive oxygen species),AOX (Antioxidants). intracellular reactive oxygen species (ROS), and cause oxidative stress in cells. The fluorescence intensity is proportional to the ROS levels. High concentrations of antioxidants can exert pro-oxidative effects. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. The ROS Detection Assay Kit ab287839 is designed for the detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Low levels of ROS play an essential role in signaling pathways, whereas increased levels under oxidative stress, results in damage to nucleic acids, proteins and membrane lipids. Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells.
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